Braakman & van der Sluijs group


McCaul ND, Quandte M, Bontjer I, van Zadelhoff G, Land A, Sanders RW and Braakman I, 2021. Intramolecular quality control: HIV-1 Envelope gp160 signal-peptide cleavage as a functional folding checkpoint. Cell Rep. 2021 36:9, 109646 Link

Sabusap CM, Joshi D, Simhaev L, Oliver KE, Senderowitz H, van Willigen M, Braakman I, Rab A, Sorscher EJ, Hong JS. The CFTR P67L variant reveals a key role for N-terminal lasso helices in channel folding, maturation, and pharmacologic rescue. J. Biol. Chem. 2021 100598 Link

Kleizen B, van Willigen M, Mijnders M, Peters FM, Grudniewska MK, Hillenaar TM, Thomas AS, Kooijman L, Peters KW, Frizzell R, van der Sluijs P, Braakman I. Co-translational folding of the first transmembrane domain of ABC-transporter CFTR is supported by assembly with the first cytosolic domain. J. Mol. Biol. 2021 13:166955 Link


Armengaud J, Delaunay‐Moisan A, Thuret JY, van Anken E, Acosta‐Alvear D, Aragón T, Arias C, Blondel M, Braakman I, Collet JF, Courcol R, Danchin A, Deleuze J‐F, Lavigne J‐P, Lucas S, Michiels T, Moore ER, Nixon‐Abell J, Rossello‐Mora R, Shi Z, Siccardi AG, Sitia R, Tillett D, Timmis KN, Toledano MB, van der Sluijs P and Vicenzi E. The Importance Of Naturally Attenuated Sars‐Cov‐2 In The Fight Against Covid‐19. Environ. Microbiol. 2020 Link

Bross P, Gersting SW, McCaffrey K, Gremme S, Muntau A, Braakman I, Gregersen N. Protein Folding and misfolding: the role of cellular protein quality control systems in inherited disorders. The Online Metabolic and Molecular Bases of Inherited Disease. Valle D, Antonarakis S, Ballabio A, Beaudet A, Mitchell GA (Ed.) McGraw-Hill Medical (2020) Chapter 13.1, DOI: 10.1036/ommbid.308 Link


Kleizen B, Hunt JF, Callebaut I, Hwang T-C, Sermet-Gaudelus I, Hafkemeyer S, Sheppard DN. CFTR: New insights into structure and function and implications for modulation by small molecules. J Cyst Fibros. 2019 19:S19-24. Link

Oliver KE, Rauscher R, Mijnders M, Wang W, Wolpert MJ, Maya J, Sabusap CM, Kesterson RA, Kirk KL, Rab A, Braakman I, Hong JS, Hartman IV JL, Ignatova Z, Sorscher EJ. Slowing ribosome velocity restores folding and function of mutant CFTR. J Clin Invest. 2019 129(12) Link

Schildknegt D, Lodder N, Pandey A, Egmond MR, Pena F, Braakman I, van der Sluijs P. Characterization of CNPY5 and its family members. Prot Sci. 2019 pro.3635 Link

McCaul N, Yeoh H Y, van Zadelhoff G, Lodder N, Kleizen B, Braakman I. Analysis of protein folding, transport, and degradation in living cells by radioactive pulse chase. JoVE. 2019 144. Link (Video article, see also Methods)

van Willigen M, Vonk AM, Yeoh HY, Kruisselbrink E, Kleizen B, van der Ent CK, Egmond MR, de Jonge HR, Braakman I, Beekman JM, van der Sluijs P. Folding-function relationship of the most common cystic fibrosis-causing CFTR conductance mutants. Life Sci Alliance. 2019 2(1): Link


Feige MJ, Braakman I, Hendershot LM. Disulfide bonds in protein folding and stability. Oxidative folding of proteins: basic principles, cellular regulation and engineering. 2018 Chapter 1.1. Link

Mall MA, Hwang TC, Braakman I. Cystic fibrosis research topics featured at the 14th ECFS Basic Science Conference: Chairman’s summary. J Cyst Fibros. 2018 17(2S):S1-S4. Link

Joshi U, Houwman JA, van der Sluijs P. Lysosomes nor mice move forward without Borcs7. Trends Cell Biol. 2018 28(10):761-763. Link

Nag S, Rani S, Mahanty S, Bissig C, Arora P, Azevedo C, Saiardi A, van der Sluijs P, Delevoye C, van Niel G, Raposo G, Setty SRG. Rab4A organizes endosomal domains for sorting cargo to lysosome-related organelles. J Cell Sci. 2018 131(18). Link

Bin NR, Ke Ma, Tien CW, Wang S, Zhu D, Park S, Turlova E, Sugita K, Shirakawa R, van der Sluijs P, Horiuchi H, Sun HS, Monnier P, Gaisano H, Sugita S. C2 Domains of Munc13-4 Are Crucial for Ca2+-Dependent Degranulation and Cytotoxicity in NK Cells. J Immunol. 2018 20:700-13doi: 10.4049/jimmunol.1800426. Link

Dhekne HS, Pylypenko O, Overeem AW, Ferreira RJ, van der Velde KJ, Rings EHHM, Posovszky C, Swertz MA, van der Sluijs P, Houdusse A, van IJzendoorn SCD. MYO5B, STX3, and STXBP2 mutations reveal a common disease mechanism that unifies a subset of congenital diarrheal disorders: A mutation update. Hum Mutat. 2018 39(3):333-44. doi: 10.1002/humu.23386. Link

Jonker CTH, Galmes R, Veenendaal T, ten Brink C, van de Welle REN, Liv N, de Rooij J, Peden A, van der Sluijs P, Mardant C, Klumperman J. Vps3 and Vps8 control integrin trafficking from early to recycling endosomes and regulate integrin-dependent functions, Nature Comm. 2018 9, doi:10.1038/s41467-018-03226-8. Link


Braakman I, Lamriben L, van Zadelhoff G, Hebert DN. Analysis of disulfide bond formation. Curr Protoc Protein Sci. 2017 90:14.1.1-14.1.21. Link

Mijnders M, Kleizen B, Braakman I. Correcting CFTR folding defects by small-molecule correctors to cure cystic fibrosis. Curr Opin Pharmacol. 2017 34:83-90. Link

Snapp EL, McCaul N, Quandte M, Cabartova Z, Bontjer I, Källgren C, Nilsson I, Land A, von Heijne G, Sanders RW, Braakman I. Structure and topology around the cleavage site regulate post-translational cleavage of the HIV-1 gp160 signal peptide. Elife. 2017 6. pii: e26067. Link

Kirchner S, Cai Z, Rauscher R, Kastelic N, Anding M, Czech A, Kleizen B, Ostedgaard LS, Braakman I, Sheppard DN, Ignatova Z. Alteration of protein function by a silent polymorphism linked to tRNA abundance. PLoS Biol. 2017 15(5):e2000779. Link

Chehab T, Criado Santos N, Holthenrich A, Koerdt SN, Disse J, Schuberth C, Reza Nazmi A., Neeft M, Koch H, Man KNM, Wojcik SM, Martin TFJ, van der Sluijs P, Brose N, Gerke V. A novel Munc13-4 / S100A10 / Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells. Mol Biol Cell. 2017 28:1688-1700. Link


van Zadelhoff G, van der Stelt M. Oxygenation of anandamide by lipoxygenases. Methods in Molecular Biology. 2016 vol 1412. Humana Press Link

McCaffrey K, Braakman I. Protein quality control at the endoplasmic reticulum. Essays Biochem. 2016 60(2):227-235. Link

Kakkar V, Kuiper EF, Pandey A, Braakman I, Kampinga HH. Versatile members of the DNAJ family show Hsp70 dependent anti-aggregation activity on RING1 mutant parkin C289G. Sci Rep. 2016 6:34830. Link

Ellgaard L, McCaul N, Chatsisvili A, Braakman I. Co- and Post-Translational Protein Folding in the ER. Traffic. 2016 17(6):615-38. Link

Shen S, Wang M, Li X, Li S, van Oers MM, Vlak JM, Braakman I, Hu Z, Deng F, Wang H. Mutational and functional analysis of N-linked glycosylation of envelope fusion protein F of Helicoverpa armigera nucleopolyhedrovirus. J Gen Virol. 2016 97(4):988-99. Link


Jevtov I, Zacharogianni M, van Oorschot MM, van Zadelhoff G, Aguilera-Gomez A, Vuillez I, Braakman I, Hafen E, Stocker H, Rabouille C. TORC2 mediates the heat stress response in Drosophila by promoting the formation of stress granules. J Cell Sci. 2015 128(14):2497-508. Link

Li X, van Oers MM, Vlak JM, Braakman I. Folding of influenza virus hemagglutinin in insect cells is fast and efficient. J Biotechnol. 2015 203:77-83. Link

Ivan V, van der Sluijs P. Methods for Analysis of AP-3/Rabip’ in Regulation of Lysosome Distribution. Methods Mol Biol. 2015 1298:245-58. Link
Erratum Link

Heijnen H, and van der Sluijs P. Platelet secretory behavior: as diverse as the granules ……or not? J Thromb Hemost. 2015 13: 2141-51. Link

Galmes R, ten Brink C, Oorschot V, Veenendaal T, Jonker C, van der Sluijs P, Klumperman J. Vps33B is required for delivery of endocytosed cargo to lysosomes. Traffic 2015 16: 1288- 1305. Link

van der Sluijs P. Early endosomal compartments. Encyclopedia of Cell Biology. 2015 192-200. Link


Mathys L, François KO, Quandte M, Braakman I, Balzarini J. Deletion of the highly conserved N-glycan at Asn260 of HIV-1 gp120 affects folding and lysosomal degradation of gp120, and results in loss of viral infectivity. PLoS One. 2014 9(6):e101181. Link
Erratum in: PLoS One. 2014 9(10):e110202. Link

Hagemeijer MC, Monastyrska I, Griffith J, van der Sluijs P, Voortman J, van Bergen en Henegouwen PM, Vonk AM, Rottier PJ, Reggiori F, de Haan CA. Membrane rearrangements mediated by coronavirus nonstructural proteins 3 and 4. Virology. 2014 458- 459:125-35. Link


Demmig-Adams B, Cohu CM, Amiard V, van Zadelhoff G, Veldink GA, Muller O, Adams WW. Emerging trade-offs – impact of photoprotectants (PsbS, xanthophylls, and vitamin E) on oxylipins as regulators of development and defense. New Phytologist. 2013 197(3):720-729 Link

Koritzinsky M, Levitin F, van den Beucken T, Rumantir RA, Harding NJ, Chu KC, Boutros PC, Braakman I, Wouters BG. Two phases of disulfide bond formation have differing requirements for oxygen. J Cell Biol. 2013 203(4):615-27. Link
Comment: Short B. A call for oxygen in the ER. J Cell Biol. 2013 203(4): 552.
Comment: Baumann K. Protein folding: A late need for oxygen. Nat Rev Mol Cell Biol 2014 15(5).

Hansen RE, Otsu M, Braakman I, Winther JR. Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells. Int J Cell Biol. 2013 2013:898563. Link

Oka OB, Pringle MA, Schopp IM, Braakman I, Bulleid NJ. ERdj5 is the ER reductase that catalyzes the removal of non-native disulfides and correct folding of the LDL receptor. Mol Cell. 2013 50(6):793-804. Link

Kleizen B, Braakman I. Cell biology. A sweet send-off. Science. 2013 340(6135):930-1. Link

Braakman I, Hebert DN. Protein folding in the endoplasmic reticulum. Cold Spring Harb Perspect Biol. 2013 5(5):a013201. Link

Benham AM, van Lith M, Sitia R, Braakman I. Ero1-PDI interactions, the response to redox flux and the implications for disulfide bond formation in the mammalian endoplasmic reticulum. Philos Trans R Soc Lond B Biol Sci. 2013 368(1617):20110403. Link

Tabak HF, Braakman I, van der Zand A. Peroxisome formation and maintenance are dependent on the endoplasmic reticulum. Annu Rev Biochem. 2013 82:723-44. Link

van der Sluijs P, Zibouche M, van Kerkhof P. Late steps in secretory lysosome exocytosis in cytotoxic lymphocytes. Frontiers Immunol. 2013 18: 359. Link


van der Zand A, Gent J, Braakman I, Tabak HF. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell. 2012 149(2):397-409. Link
Comment: Schuldt A. Organelle biogenesis: When two become one. Nat Rev Mol Cell Biol. 2012 13(6): 337.
Comment: Goodman C. Cell biology: Peroxisomes come together. Nat Chem Biol. 2012 8: 503.

Elstak ED, Neeft M, Nehme NT, Callebaut I, de Saint Basile G, van der Sluijs P. Munc13-4*rab27 complex tethers secretory lysosomes at the plasma membrane. Commun Integr Biol. 2012 5: 64-7. Link

Ivan V, Martinez Sanchez E, Sima L, Oorschot V, Klumperman J, Petrescu SM, van der Sluijs P. AP-3 and rabip4’ coordinately regulate spatial distribution of lysosomes. PLoS One. 2012 7: e48142. Link


Peters KW, Okiyoneda T, Balch WE, Braakman I, Brodsky JL, Guggino WB, Penland CM, Pollard HB, Sorscher EJ, Skach WR, Thomas PJ, Lukacs GL, Frizzell RA. CFTR Folding Consortium: methods available for studies of CFTR folding and correction. Methods Mol Biol. 2011 742:335-53. Link

Braakman I, Bulleid NJ. Protein folding and modification in the mammalian endoplasmic reticulum. Annu Rev Biochem. 2011 80:71-99. Link

Elstak ED, Neeft M, Nehme NT, Voortman J, Cheung M, Goodarzifard M, Gerritsen HC, van Bergen en Henegouwen PMP, Callebaut, I, de Saint Basile G, van der Sluijs P. The Munc13-4 rab27 complex is specifically required for tethering secretory lysosomes at the plasma membrane. Blood. 2011 118: 1570-8. Link

Elstak ED, te Loo M, Tesselaar K,van Kerkhof P, Loeffen J, Grivas D, Hennekam E, Boelens JJ, Hoogerbrugge PM, van der Sluijs P, van Gijn ME, van de Corput L. A novel Dutch mutation in UNC13D reveals an essential role of the C2B domain in munc13-4 function. Ped Blood Cancer. 2011 58: 598-605. Link

Grigoriev I, Yu KL, Martinez-Sanchez E, Serra Marques AA, Keijzer N, Demmers J, Peränen J, Pasterkamp RJ, van der Sluijs P, Hoogenraad CC, and Akhmanova A. Rab6, Rab8 and MICAL3 cooperate in controlling docking and fusion of exocytotic carriers. Curr Biol. 2011 21: 967-74. Link

van der Sluijs P, Hoogenraad CC. New insights in endosomal dynamics and AMPA receptor trafficking. Semin Cell Dev Biol. 2011 22: 499-505. Link

Elstak EDR, de Jong A, van der Sluijs P. A platform for complementation and characterization of familial haemophagocytic lymphohistiocytosis 3 mutations. J Immunol Meth. 2011 365: 58-66. Link


Amadio D, Fezza F, Catanzaro G, Incani O, van Zadelhoff G, Agro AF, Maccarrone M. Methylation and acetylation of 15-hydroxyanandamide modulate its interaction with the endocannabinoid system. Biochimie 2010 92(4):378-387 Link

Hoelen H, Kleizen B, Schmidt A, Richardson J, Charitou P, Thomas PJ, Braakman I. The primary folding defect and rescue of ΔF508 CFTR emerge during translation of the mutant domain. PLoS One. 2010 5(11):e15458. Link

van der Zand A, Braakman I, Tabak HF. Peroxisomal membrane proteins insert into the endoplasmic reticulum. Mol Biol Cell. 2010 21(12):2057-65. Link

Christis C, Fullaondo A, Schildknegt D, Mkrtchian S, Heck AJ, Braakman I. Regulated increase in folding capacity prevents unfolded protein stress in the ER. J Cell Sci. 2010 123(Pt 5):787-94. Link

Pena F, Jansens A, van Zadelhoff G, Braakman I. Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum. J Biol Chem. 2010 285(12):8656-64. Link

Balch WE, Braakman I, Frizzell R, Guggino W, Lukacs G, Penland C, Skach W, Sorscher E, Thomas P, Pollard H. The folding biology of cystic fibrosis: a consortium-based approach to disease. Protein misfolding diseases : current and emerging principles and therapies. 2010. Chapter 19. Link

Fraile-Ramos A, Cepeda V, Elstak EDR, van der Sluijs P. Rab27a is required for human cytomegalovirus assembly. PLoS One 2010 5: e15318. Link

Martinez Sanchez E, Popa I, Deneka M, van der Sluijs P. Role of rab4 in transport through the endosomal system. Rab GTPases and Membrane Trafficking 2010. 108-22 Link

Hoogenraad CC, van der Sluijs P. GRASP-1 regulates endocytic receptor recycling and synaptic plasticity. Commun Integr Biol. 2010 3:5, 1-3. Link

Ivan V, van der Sluijs P. Sorting and transport of melanosomal enzymes between post Golgi compartments. Rom J Biochem. 2010 47: 179-91.

Hoogenraad CC, Popa I, Futai K, Sanchez-Martinez E, Wulf PS, van Vlijmen T, Dortland BR, Oorschot V, Govers R, Monti M, Heck AJR, Sheng M, Klumperman J, Rehmann H, Jaarsma D, Kapitein LC, van der Sluijs P. Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes. PLoS Biol. 2010 8: e1000283. Link


van Anken E, Pena F, Hafkemeijer N, Christis C, Romijn EP, Grauschopf U, Oorschot VM, Pertel T, Engels S, Ora A, Lástun V, Glockshuber R, Klumperman J, Heck AJ, Luban J, Braakman I. Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1. Proc Natl Acad Sci USA. 2009 106(40):17019-24. Link

Braakman I. Entering a new era with Ero. Nat Rev Mol Cell Biol. 2009 10(8):503. Link

Bontjer I, Land A, Eggink D, Verkade E, Tuin K, Baldwin C, Pollakis G, Paxton WA, Braakman I, Berkhout B, Sanders RW. Optimization of human immunodeficiency virus type 1 envelope glycoproteins with V1/V2 deleted, using virus evolution. J Virol. 2009 83(1):368-83. Link

Kloer DP, Rojas R, Ivan V, Moriyama K, van Vlijmen T, Ghirlando R, van der Sluijs P, Hurley JH, Bonifacino JS. Assembly of the biogenesis of lysosome-related organelles complex-3 (BLOC-3) and its interaction with Rab9. J Biol Chem. 2009 285: 7794-8004. Link

Savelkoul PJ, De Mattia F, Li Y, Kamsteeg EJ, Konings IB, van der Sluijs P, Deen PM. P.R254Q mutation in the aquaporin-2 water channel causing dominant nephrogenic diabetes insipidus is due to a lack of arginine vasopressin-induced phosphorylation. Hum Mutat. 2009 10: 681-93. Link

van Balkom BWM, Boone M, Hendriks G, Kamsteeg EJ, van der Voorde A, van Herp F, van der Sluijs P, Deen PMT. LIP5 interacts with aquaporin 2 and facilitates its lysosomal degradation. J Am Soc Nephrol. 2009 20: 990-1001. Link


Sanders RW, Hsu ST, van Anken E, Liscaljet IM, Dankers M, Bontjer I, Land A, Braakman I, Bonvin AM, Berkhout B. Evolution rescues folding of human immunodeficiency virus-1 envelope glycoprotein GP120 lacking a conserved disulfide bond. Mol Biol Cell. 2008 19(11):4707-16. Link

Christis C, Lubsen NH, Braakman I. Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol. FEBS J. 2008 275(19):4700-27. Link

Braakman I, Otsu M. Biochemistry. Cargo load reduction. Science. 2008 321(5888):499-500. Link

van Anken E, Sanders RW, Liscaljet IM, Land A, Bontjer I, Tillemans S, Nabatov AA, Paxton WA, Berkhout B, Braakman I. Only five of 10 strictly conserved disulfide bonds are essential for folding and eight for function of the HIV-1 envelope glycoprotein. Mol Biol Cell. 2008 19(10):4298-309. Link

Tabak HF, van der Zand A, Braakman I. Peroxisomes: minted by the ER. Curr Opin Cell Biol. 2008 20(4):393-400. Link

Sanders RW, van Anken E, Nabatov AA, Liscaljet IM, Bontjer I, Eggink D, Melchers M, Busser E, Dankers MM, Groot F, Braakman I, Berkhout B, Paxton WA. The carbohydrate at asparagine 386 on HIV-1 gp120 is not essential for protein folding and function but is involved in immune evasion. Retrovirology. 2008 5:10. doi: 10.1186/1742-4690-5-10. Link

Rojas R, van Vlijmen T, Mardones GA, Prabhu Y, Rojas AL, Mohammed S, Heck AJR, Raposo G, van der Sluijs P, Bonifacino JS. Regulation of retromer recruitment to endosomes by sequential action of rab5 and rab7. J Cell Biol. 2008 183: 513-26. Link

van Vlijmen T, Vleugel M, Evers N, Mohammed S, Wulf P, Heck AJR, Hoogenraad C, van der Sluijs P. A unique residue in rab3 determines the interaction with novel binding protein Zwint-I. FEBS Lett. 2008 582: 2838-42. Link

Fila C, Metz C, van der Sluijs P. Juglone inactivates cysteine-rich proteins required for progression through mitosis. J Biol Chem. 2008 283: 21714-24. Link

Ahmed BA, Jeffus BC, Bukhari SI, Harney JT, Unal R, Lupashin VV, van der Sluijs P, Kilic F. Serotonin transamidates rab4 and facilitates its binding to the C terminus of serotonin transporter. J Biol Chem. 2008 283: 9388-98. Link

van der Sluijs P, Neeft M, van Vlijmen T, Elstak E, Wieffer M. Methods for analysis of rab27a/munc13-4 in secretory lysosome release in haematopoietic cells. Meth Enzymol. 2008 438: 185-201. Link

Kamsteeg EJ, Savelkoul PJ, Hendriks G, Konings IB, Nivillac NM, Lagendijk AK, van der Sluijs P, Deen PM. Missorting of the Aquaporin-2 mutant E258K to multivesicular bodies/lysosomes in dominant NDI is associated with its monoubiquitination and increased phosphorylation by PKC but is due to the loss of E258. Pflugers Arch. 2008 455: 1041-54. Link


Tabak HF, Hoepfner D, van der Zand A, Geuze HJ, Braakman I, Huynen MA. Formation of peroxisomes: present and past. Biochim Biophys Acta. 2006 1763(12):1647-54. Link

van der Zand A, Braakman I, Geuze HJ, Tabak HF. The return of the peroxisome. J Cell Sci. 2006 119(Pt 6):989-94. Link

De Jonge W, Tabak HF, Braakman I. Chaperone proteins and peroxisomal protein import. Top Curr Genet – Chaperones. 2006 Chapter 6:149-83. Link

Piersma D, Berns EM, Verhoef-Post M, Uitterlinden AG, Braakman I, Pols HA, Themmen AP. A common polymorphism renders the luteinizing hormone receptor protein more active by improving signal peptide function and predicts adverse outcome in breast cancer patients. J Clin Endocrinol Metab. 2006 91(4):1470-6. Link

Linder MD, Uronen RL, Vuori MH, van der Sluijs P, Peränen J, Ikonen E. Rab8- dependent recycling promotes endosomal cholesterol removal in normal and sphingolipidosis cells. Mol Biol Cell. 2006 18, 47-56. Link

Kamsteeg EJ, Hendriks G, Konings IBM, Oorschot V, van der Sluijs P, Klumperman J, Deen PMT. Short-chain ubiquitination mediates the regulated endocytosis of the aquaporin-2 water channel. Proc Natl Acad Sci USA. 2006 103, 18344-9. Link


van Anken E, Braakman I. Endoplasmic reticulum stress and the making of a professional secretory cell. Crit Rev Biochem Mol Biol. 2005 40(5):269-83. Link

Kleizen B, van Vlijmen T, de Jonge HR, Braakman I. Folding of CFTR is predominantly cotranslational. Mol Cell. 2005 20(2):277-87. Link

van Anken E, Braakman I. Versatility of the endoplasmic reticulum protein folding factory. Crit Rev Biochem Mol Biol. 2005 40(4):191-228. Link

Hoepfner D, Schildknegt D, Braakman I, Philippsen P, Tabak HF. Contribution of the endoplasmic reticulum to peroxisome formation. Cell. 2005 122(1):85-95. Link
Comment: Schekman R. Peroxisomes: another branch of the secretory pathway? Cell 2005 122:1-2.
Comment: Kunau WH. Peroxisome biogenesis: end of the debate. Curr Biol. 2005 15(18): 774-R776.

Romijn EP, Christis C, Wieffer M, Gouw JW, Fullaondo A, van der Sluijs P, Braakman I, Heck AJ. Expression clustering reveals detailed co-expression patterns of functionally related proteins during B cell differentiation: a proteomic study using a combination of one-dimensional gel electrophoresis, LC-MS/MS, and stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics. 2005 4(9):1297-310. Link

Maggioni MC, Liscaljet IM, Braakman I. A critical step in the folding of influenza virus HA determined with a novel folding assay. Nat Struct Mol Biol. 2005 12(3):258-63.Link

Maggioni C, Braakman I. Synthesis and quality control of viral membrane proteins. Curr Top Microbiol Immunol. 2005 285:175-98. Link

Liscaljet IM, Kleizen B, Braakman I. Studying protein folding in vivo. Protein Folding Handbook. 2005 Part II, Volume 1, Chapter 3: 73-104. Link

de Mattia F, Savelkoul PJM, Kamsteeg, EJ, Konings IBM, van der Sluijs P, Mallmann R, Oksche A, Deen PMT. Lack of arginine vasopressin-induced phosphorylation of Aquaporin-2 mutant AQP2-R254L explains dominant Nephrogenic Diabetes Insipidus. J Am Soc Nephrol. 2005 16, 2872-80. Link

Popa I, Deneka M, van der Sluijs P. Expression and properties of the Rab4, Rabaptin-5alpha, AP-1 complex in endosomal recycling. Meth Enzymol. 2005 403, 526-40. Link

Romijn EP, Christis C, Wieffer M, Gouw JW, Fullaondo A, van der Sluijs P, Braakman I, Heck AJ. Expression clustering reveals detailed co-expression patterns of functionally related proteins during B cell differentiation: a proteomic study using a combination of one-dimensional gel electrophoresis, LC-MS/MS, and stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics. 2005 4(9):1297-310. Link


Gent J, Braakman I. Low-density lipoprotein receptor structure and folding. Cell Mol Life Sci. 2004 61(19-20):2461-70. Link

Kleizen B, Braakman I. Protein folding and quality control in the endoplasmic reticulum. Curr Opin Cell Biol. 2004 16(4):343-9. Link
Erratum in: Curr Opin Cell Biol. 2004 16(5):597. Link

Neeft M, Wieffer M, de Jong AS, Negroiu G, Metz C, van Loon A, Krijgsveld J, Griffith J, Wulffraat N, Koch H, Heck A, Brose N, Kleijmeer M, van der Sluijs P. Munc13-4 is an effector of rab27a and controls secretion of lysosomes in haematopoietic cells. Mol Biol Cell 2004 15, 731-41. Link

de Mattia F, Savelkoul PJM, Bichet, D, Kamsteeg, EJ, Konings IBM, Marr, N, Arthus MF, Lonergan MF, van Os, CH, van der Sluijs P, Robertson G, Deen PMT. A novel mechanism in recessive Nephrogenic Diabetes Insipidus: wild-type Aquaporin-2 rescues the apical membrane expression of intracellularly retained AQP2-P262L. Hum Mol Genet. 2004 13, 3045-56. Link

de Graaf P, van Dijken RAJ, Zwart W.T. Deneka M, Schulz TKF, Geijsen N, Coffer PJ, Gadella BM, Verkleij AJ, van der Sluijs P, van Bergen en Henegouwen PMP. Phosphatidylinositol-4-OHkinasebeta binds to and is critical for rab11-association with the Golgi complex. Mol Biol Cell 2004 15, 2038-47. Link

van Balkom BW, Graat MP, van Raak M, Hofman E, van der Sluijs P, Deen PM. Role of cytoplasmic termini in sorting and shuttling of the aquaporin-2 water channel. Am J Physiol Cell Physiol. 2004 286(2):C372-9. Link

Roberts M, Woods AJ, van der Sluijs P, Norman JC. Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins. Mol Cell Biol. 2004 24, 1505-15. Link

Hendriks G, Koudijs M, van Balkom BWM, Oorschot V, Klumperman J, Deen PMT, van der Sluijs P. Glycosylation is essential for cell surface expression of the water channel aquaporin-2, but not for its tetramerization in the endoplasmic reticulum. J Biol Chem. 2004 279, 2975-83. Link

Fouraux MA, Deneka M, Ivan V, van der Heijden A, Raymackers J, van Suylekom D, van Venrooij WJ, van der Sluijs P, Pruijn GJM. rabip4′ is an effector of rab5 and rab4 and regulates transport through early endosomes. Mol Biol Cell 2004 15, 611-24. Link


Sitia R, Braakman I. Quality control in the endoplasmic reticulum protein factory. Nature. 2003 426(6968):891-4. Link

van der Vlies D, Makkinje M, Jansens A, Braakman I, Verkleij AJ, Wirtz KW, Post JA. Oxidation of ER resident proteins upon oxidative stress: effects of altering cellular redox/antioxidant status and implications for protein maturation. Antioxid Redox Signal. 2003 5(4):381-7. Link

Jansens A, Braakman I. Pulse-chase labeling techniques for the analysis of protein maturation and degradation. Methods Mol Biol. 2003 232:133-45. Link

Tabak HF, Murk JL, Braakman I, Geuze HJ. Peroxisomes start their life in the endoplasmic reticulum. Traffic. 2003 4(8):512-8. Link

Land A, Zonneveld D, Braakman I. Folding of HIV-1 envelope glycoprotein involves extensive isomerization of disulfide bonds and conformation-dependent leader peptide cleavage. FASEB J. 2003 17(9):1058-67. Link

van Anken E, Romijn EP, Maggioni C, Mezghrani A, Sitia R, Braakman I, Heck AJ. Sequential waves of functionally related proteins are expressed when B cells prepare for antibody secretion. Immunity. 2003 18(2):243-53. Link

Deneka M, Neeft M, Popa I, van Oort M, Sprong H, Oorschot V, Klumperman J, Schu P, van der Sluijs P. rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and g1-adaptin in membrane recycling from endosomes. EMBO J. 2003 22, 2645-57. Link

Sprong H, Degroote S, Nilsson T, Kawakita M, Ishida N, van der Sluijs P, van Meer G. Association of the UDP-galactose transporter with UDP-galactose:ceramide galactosyltransferase allows UDP-galactose import in the endoplasmic reticulum. Mol Biol Cell 2003 14, 3482-93. Link

Deneka M, Neeft M, van der Sluijs P. Regulation of membrane transport by rab GTPases. Crit Rev Biochem Mol Biol. 2003 38, 121-42. Link

van Balkom BWM, van Raak M, Breton S, Pastor-Soler N, Bouley R, van der Sluijs P, Brown D, Deen PMT. Hypertonicity is involved in redirecting the aquaporin-2 water channel into the basolateral, instead of the apical, plasma membrane of renal epithelial cells. J Biol Chem. 2003 278, 1101-7. Link


Jansens A, van Duijn E, Braakman I. Coordinated nonvectorial folding in a newly synthesized multidomain protein. Science. 2002 298(5602):2401-3. Link

van Balkom BWM, Savekoul PJM, Markovich D, Hofman E, Nielsen S, van der Sluijs P, Deen PMT. The role of putative phosphorylation sites in the targeting and shuttling of the aquaporin-2 water channel. J Biol Chem. 2002 277, 41473-9. Link

Nellist M, Goedbloed MA, de Winter C, Verhaaf B, Jankie A, Reuser AJJ, van den Ouweland AMW, van der Sluijs P, Halley DJJ. Identification and characterization of the interaction between tuberin and 14-3-3zeta. J Biol Chem. 2002 277, 39417-24. Link

Mohrmann K, Gerez L, Oorschot V, Klumperman J, van der Sluijs P. Rab4 function in membrane recycling from early endosomes depends on a membrane to cytoplasm cycle. J Biol Chem. 2002 277, 32029- 35. Link

Mohrmann K, Leijendekker R, Gerez L, van der Sluijs P. Rab4 regulates transport to the apical plasma membrane in Madin-Darby Canine Kidney cells. J Biol Chem. 2002 277: 10474-81. Link

Govers R, van der Sluijs P, Donselaar E, Slot JW, Rabelink TJ. Endothelial nitric oxide synthase and its negative regulator caveolin-1 localize to distinct perinulear organelles. J Histochem Cytochem. 2002 50: 779-88. Link

Deneka M, van der Sluijs P. ‘Rab’ing up endosomal membrane transport. Nature Cell Biol. 2002 4, E32-5. Link


Mezghrani A, Fassio A, Benham A, Simmen T, Braakman I, Sitia R. Manipulation of oxidative protein folding and PDI redox state in mammalian cells. EMBO J. 2001 20(22):6288-96. Link

Land A, Braakman I. Folding of the human immunodeficiency virus type 1 envelope glycoprotein in the endoplasmic reticulum. Biochimie. 2001 83(8):783-90. Link

Braakman I. A novel lectin in the secretory pathway. An elegant mechanism for glycoprotein elimination. EMBO Rep. 2001 2(8):666-8. Link

Braakman I, Hebert DN. Analysis of disulfide bond formation. Curr Protoc Protein Sci. 2001 Chapter 14:Unit14.1. Link

Sprong H, Hirabayashi Y, Westerink B, Oorschot V, Klumperman J, van der Sluijs P, van Meer G. Ceramide glucosyltransferase is required in sorting tyrosinase from the Golgi complex to melanosomes. J Cell Biol. 2001 155: 369-79. Link

Roberts M, Barry S, Woods A, van der Sluijs P, Norman J. PDGF-regulated rab4-dependent recycling of alphavbeta3 integrin from early endosomes is necessary for cell adhesion and spreading. Curr. Biol. 2001 11: 1392-1402. Link

de Wit H, Lichtenstein Y, Kelly R, Geuze HJ, Klumperman J, van der Sluijs P. Rab4 enhances synaptic vesicle formation from early endosomes in PC12 cells. Mol Biol Cell 2001 12: 3703-15. Link

Sprong H, van der Sluijs P, van Meer G. How proteins move lipids and lipids move proteins. Nature Revs Mol Cell Biol. 2001 2: 504-14. Link

Deneka M, Negroiu G, van der Sluijs P. Pigmentation defects uncover a new function for rabs in organelle transport: rab27a in organelle motility. ELSO Gazette #4, 2001


Benham AM, Braakman I. Glycoprotein folding in the endoplasmic reticulum. Crit Rev Biochem Mol Biol. 2000 35(6):433-73. Link

Braakman I, van Anken E. Folding of viral envelope glycoproteins in the endoplasmic reticulum. Traffic. 2000 1(7):533-9. Link

Kleizen B, Braakman I, de Jonge HR. Regulated trafficking of the CFTR chloride channel. Eur J Cell Biol. 2000 79(8):544-56. Link

Benham AM, Cabibbo A, Fassio A, Bulleid N, Sitia R, Braakman I. The CXXCXXC motif determines the folding, structure and stability of human Ero1-Lα. EMBO J. 2000 19(17):4493-502. Link

Distel B, Braakman I, Elgersma Y, Tabak HF. Transactions at the peroxisomal membrane. Subcellular Biochemistry – Fusion of biological membranes and related problems. 2000 Chapter 8:303-22. Link

Ermonval M, Duvet S, Zonneveld D, Cacan R, Buttin G, Braakman I. Truncated N-glycans affect protein folding in the ER of CHO-derived mutant cell lines without preventing calnexin binding. Glycobiology. 2000 10(1):77-87. Link

van der Sluijs P, Mohrmann K, Deneka M, Jongeneelen M. Expression and properties of rab4 and its effector rabaptin-4 in endocytic recycling. Meth Enzymol. 2000 329: 111-9. Link

Gerez L, Mohrmann K, van Raak M, Lu KP, van der Sluijs P. Accumulation of rab4GTP in the Cytoplasm and Association with the Peptidyl-Prolyl Isomerase Pin1 during Mitosis. Mol Biol Cell 2000 11: 2201-11. Link

Nagelkerken B, van Anken E, Mohrmann K, Gerez L, van Raak M, van Uden N, Holthuizen J, Pelkmans L, van der Sluijs P. Rabaptin4, a novel effector of the small GTPase rab4a, is recruited to perinuclear recycling vesicles. Biochem J. 2000 346: 593-601. Link

Scheper W, Zwart R, van der Sluijs P, Annaert W, van Gool WA, Baas, F. Alzheimer’s PS1 is a putative membrane receptor for rab GDP dissociation inhibitor. Hum Mol Gen. 2000 9: 303-10. Link


Das AT, Land A, Braakman I, Klaver B, Berkhout B. HIV-1 evolves into a nonsyncytium-inducing virus upon prolonged culture in vitro. Virology. 1999 263(1):55-69. Link

Tabak HF, Braakman I, Distel B. Peroxisomes: simple in function but complex in maintenance. Trends Cell Biol. 1999 9(11):447-53. Link

Sprong H, van Meer G, van der Sluijs P. Analysis of galactolipids and UDP-galactose: ceramide galactosyltransferase. Meth Enzymol. 1999 311: 59-73. Link

Nellist M, Slegtenhorst M, van den Ouweland A, Halley D, van der Sluijs P. Characterization of the cytosolic tuberin-hamartin complex. Tuberin is a cytosolic chaperone for hamartin. J Biol Chem. 1999 274: 35647-52. Link

de Wit H, Lichtenstein Y, Kelly R, van der Sluijs P, Klumperman J. Synaptic Vesicles Form by Budding from Tubular Extensions of Sorting Endosomes in PC12 Cells. Mol Biol Cell 1999 10: 4163-76. Link

van der Sluijs P, Gerez, L. Rab GTPases as regulators of transport through endosomes. Protoplasma 1999 210: 1-10. Link

Mohrmann K, van der Sluijs P. Regulation of membrane transport through the endocytic pathway by rab GTPases. Mol Membr Biol. 1999 16: 81-8. Link


Hettema EH, Ruigrok CC, Koerkamp MG, van den Berg M, Tabak HF, Distel B, Braakman I. The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import. J Cell Biol. 1998 142(2):421-34. Link

van Weering DH, Moen TC, Braakman I, Baas PD, Bos JL. Expression of the receptor tyrosine kinase Ret on the plasma membrane is dependent on calcium. J Biol Chem. 1998 273(20):12077-81. Link

Slegtenhorst M, Nellist M, Nagelkerken B, Cheadle J, Snell R, van den Ouweland A, Reuser A, Sampson J, Halley D, van der Sluijs P. Interactions between hamartin and tuberin, the TSC1 and TSC2 gene products. Hum Mol Gen. 1998 7:1053-7. Link

Sprong H, Kruithof B, Leijendekker R, Slot JW, Van Meer G, van der Sluijs P. UDP-Galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum. J Biol Chem. 1998 273: 25880-8. Link

Mulders SM, Bichet DG, Rijs JPL, Kamsteeg EJ, Arthus MF, Lonergan M, Fujiwara M, Morgan K, Leijendekker RLL, van der Sluijs P, van Os CH, Deen PMT. An aquaporin-2 water channel mutant which causes autosomal nephrogenic diabetes insipidus is retained in the Golgi complex. J Clin Invest. 1998 102: 57-66. Link


Zhang JX, Braakman I, Matlack KE, Helenius A. Quality control in the secretory pathway: the role of calreticulin, calnexin and BiP in the retention of glycoproteins with C-terminal truncations. Mol Biol Cell. 1997 8(10):1943-54. Link

Chavrier P, van der Sluijs P, Nagelkerken B, Gorvel JP. Early endosome membrane dynamics characterized by flow cytometry. Cytometry 1997 29(1): 41-9. Link

Nagelkerken B, Mohrmann K, Gerez L, Van Raak M, Leijendekker R, van der Sluijs P. A novel epitope tag for the detection of rab GTPases. Electrophoresis 1997 18(14): 2694-8. Link


Bottger G, Nagelkerken B, van der Sluijs P. Rab4 and rab7 define distinct, nonoverlapping endosomal compartments. J Biol Chem. 1996 271(46): 29191-7. Link

Elgersma Y, Vos A, van den Berg M, Roermund CWT, van der Sluijs P, Distel B, Tabak HF. Analysis of the carboxyterminal peroxisomal targeting signal in a homologous context in Saccharomyces cerevisiae. J Biol Chem 1996. 271(42): 26375-82. Link

Daro E, van der Sluijs P, Galli T, Mellman I. Rab4 and cellubrevin define different early endosome populations on the pathway of transferrin receptor recycling. Proc Natl Acad Sci USA. 1996 93(18): 9559-64. Link


Chen W, Helenius J, Braakman I, Helenius A. Cotranslational folding and calnexin binding during glycoprotein synthesis. Proc Natl Acad Sci USA. 1995 92(14):6229-33. Link


Hammond C, Braakman I, Helenius A. Role of N-linked oligosaccharide recognition, glucose trimming, and calnexin in glycoprotein folding and quality control. Proc Natl Acad Sci USA. 1994 91(3):913-7. Link

van der Sluijs P. The small GTPase rab4 and membrane transport. GTPase controlled molecular machines. 1994 271-83.


Tatu U, Braakman I, Helenius A. Membrane glycoprotein folding, oligomerization and intracellular transport: effects of dithiothreitol in living cells. EMBO J. 1993 12(5):2151-7. Link

de Silva A, Braakman I, Helenius A. Posttranslational folding of vesicular stomatitis virus G protein in the ER: involvement of noncovalent and covalent complexes. J Cell Biol. 1993 120(3):647-55. Link

Helenius A, Tatu U, Marquardt T, Braakman I. Protein Folding in the Endplasmic Reticulum. Cell Biology and Biotechnology. 1993 Chapter 10: 125-36. Link

van der Sluijs P, Hull M, Mellman I. The small GTPase Rab4 controls an early endocytic sorting event. Molecular mechanisms of membrane transport. 1993 399-402. Link


Helenius A, Marquardt T, Braakman I. The endoplasmic reticulum as a protein-folding compartment. Trends Cell Biol. 1992 2(8):227-31. Link

Braakman I, Helenius J, Helenius A. Manipulating disulfide bond formation and protein folding in the endoplasmic reticulum. EMBO J. 1992 11(5):1717-22. Link

Braakman I, Helenius J, Helenius A. Role of ATP and disulphide bonds during protein folding in the endoplasmic reticulum. Nature. 1992 356(6366):260-2. Link

van der Sluijs P, Hull M, Webster P, Male P, Goud B, Mellman I. The small GTP binding protein rab4 controls an early sorting event on the endocytic pathway. Cell 1992 70(5):729-40. Link

van der Sluijs P, Hull M, Huber L, Male P, Goud B, Mellman I. Reversible phosphorylation-dephosphorylation determines the localization of rab4 during the cell cycle. EMBO J. 1992 11(12): 4379-89. Link


Braakman I, Hoover-Litty H, Wagner KR, Helenius A. Folding of influenza hemagglutinin in the endoplasmic reticulum. J Cell Biol. 1991 114(3):401-11. Link

Braakman I, Keij J, Hardonk MJ, Meijer DK, Groothuis GM. Separation of periportal and perivenous rat hepatocytes by fluorescence-activated cell sorting: confirmation with colloidal gold as an exogenous marker. Hepatology. 1991 13(1):73-82. Link

van der Sluijs P, Hull M, Zahraoui A, Tavitian A, Goud B, Mellman I. The small GTP-binding protein rab4 is associated with early endosomes. Proc Natl Acad Sci. USA. 1991 88:6313-7. Link

van der Sluijs P, Meijer DKF. Limitations on the specificity of targeting asialoglycoprotein conjugates to the liver. Targeted diagnosis of liver diseases: cell surface receptors and directed agents. 1991 Chapter 11:235-64.


van der Sluijs P, Bennett MK, Anthony C, Simons K, Kreis TE. Binding of exocytic vesicles from MDCK cells to microtubules in vitro. J Cell Sci. 1990 95:545-53. Link


Thalhammer T, Gessl A, Braakman I, Graf J. Separation of hepatocytes of different acinar zones by flow cytometry. Cytometry. 1989 10(6):772-8. Link

Braakman I, Pijning T, Verest O, Weert B, Meijer DK, Groothuis GM. Vesicular uptake system for the cation lucigenin in the rat hepatocyte. Mol Pharmacol. 1989 36(4):537-42. Link

Braakman I, Verest O, Pijning T, Meijer DK, Groothuis GM. Zonal distribution of the cation lucigenin in rat liver: influence of taurocholate. Mol Pharmacol. 1989 36(4):532-6. Link

Braakman I, Groothuis GM, Meijer DK. Zonal compartmentation of perfused rat liver: plasma reappearance of rhodamine B explained. J Pharmacol Exp Ther. 1989 249(3):869-73. Link

Meijer DKF, van der Sluijs P. Binding of drugs to alpha-1-acid glycoprotein and its desialylated form. Influence on hepatic disposition an implications for drug targeting to the liver. Progr Clin Biol Res. 1989 300:143-67.

Meijer DKF, van der Sluijs P. Covalent and noncovalent binding of drugs: implications for hepatic clearance, storage and cell-specific drug delivery. Pharm Res. 1989 6(2):105-18. Link


van der Sluijs P, Braakman I, Meijer DK, Groothuis GM. Heterogeneous acinar localization of the asialoglycoprotein internalization system in rat hepatocytes. Hepatology. 1988 8(6):1521-9. Link


Braakman I, Groothuis GM, Meijer DK. Acinar redistribution and heterogeneity in transport of the organic cation rhodamine B in rat liver. Hepatology. 1987 7(5):849-55. Link

Meijer DKF, van der Sluijs P. The influence of binding to albumin and alpha-1-acid glycoprotein on the clearance of drugs by the liver. Pharm Weekbl, Sci Ed. 1987 9(2): 65-74. Link

van der Sluijs P, Spanjer HH, Meijer DKF. Hepatic disposition of cationic drugs bound to asialoorosomucoid: lack of co-endocytosis and evidence for intrahepatic dissociation. J Pharmacol Exp Ther. 1987 240(2): 668-73. Link

van der Sluijs P, Postema B, Meijer DKF. Lactosylation of albumin reduces the uptake rate of dibromosulphthalein in perfused liver, and dissociation rate from albumin in vitro. Hepatology 1987 7(4): 688-95. Link


van der Sluijs P, Bootsma HP, Postema B, Moolenaar F, Meijer DKF. Drug targeting to the liver with lactosylated albumins: does the glycoprotein target the drug or is the drug targeting the glycoprotein. Hepatology 1986 6(4):723-8. Link


van der Sluijs P, Meijer DKF. Binding of drugs with a quaternary ammonium group to alpha-1-acid glycoprotein and asialo alpha-1-acid glycoprotein. J Pharmacol Exp Ther. 1985 234(3): 703-7. Link

van der Sluijs P, Oosting R, Weitering JG, Hardonk MJ, Meijer DKF. Biliary excretion of FITC metabolites after administration of FITC labeled asialoorosomucoid as a measure of lysosomal proteolysis. Biochem Pharmacol. 1985 34(9):1399-405. Link