Braakman group


Feige MJ, Braakman I, Hendershot LM. Disulfide bonds in protein folding and stability. Oxidative folding of proteins: basic principles, cellular regulation and engineering. 2018 Chapter 1.1. Link

Mall MA, Hwang TC, Braakman I. Cystic fibrosis research topics featured at the 14th ECFS Basic Science Conference: Chairman’s summary. J Cyst Fibros. 2018 17(2S):S1-S4. Link


Braakman I, Lamriben L, van Zadelhoff G, Hebert DN. Analysis of disulfide bond formation. Curr Protoc Protein Sci. 2017 90:14.1.1-14.1.21. Link

Mijnders M, Kleizen B, Braakman I. Correcting CFTR folding defects by small-molecule correctors to cure cystic fibrosis. Curr Opin Pharmacol. 2017 34:83-90. Link

Snapp EL, McCaul N, Quandte M, Cabartova Z, Bontjer I, Källgren C, Nilsson I, Land A, von Heijne G, Sanders RW, Braakman I. Structure and topology around the cleavage site regulate post-translational cleavage of the HIV-1 gp160 signal peptide. Elife. 2017 6. pii: e26067. Link

Kirchner S, Cai Z, Rauscher R, Kastelic N, Anding M, Czech A, Kleizen B, Ostedgaard LS, Braakman I, Sheppard DN, Ignatova Z. Alteration of protein function by a silent polymorphism linked to tRNA abundance. PLoS Biol. 2017 15(5):e2000779. Link


McCaffrey K, Braakman I. Protein quality control at the endoplasmic reticulum. Essays Biochem. 2016 60(2):227-235. Link

Kakkar V, Kuiper EF, Pandey A, Braakman I, Kampinga HH. Versatile members of the DNAJ family show Hsp70 dependent anti-aggregation activity on RING1 mutant parkin C289G. Sci Rep. 2016 6:34830. Link

Ellgaard L, McCaul N, Chatsisvili A, Braakman I. Co- and Post-Translational Protein Folding in the ER. Traffic. 2016 17(6):615-38. Link

Shen S, Wang M, Li X, Li S, van Oers MM, Vlak JM, Braakman I, Hu Z, Deng F, Wang H. Mutational and functional analysis of N-linked glycosylation of envelope fusion protein F of Helicoverpa armigera nucleopolyhedrovirus. J Gen Virol. 2016 97(4):988-99. Link


Jevtov I, Zacharogianni M, van Oorschot MM, van Zadelhoff G, Aguilera-Gomez A, Vuillez I, Braakman I, Hafen E, Stocker H, Rabouille C. TORC2 mediates the heat stress response in Drosophila by promoting the formation of stress granules. J Cell Sci. 2015 128(14):2497-508. Link

Li X, van Oers MM, Vlak JM, Braakman I. Folding of influenza virus hemagglutinin in insect cells is fast and efficient. J Biotechnol. 2015 203:77-83. Link


Mathys L, François KO, Quandte M, Braakman I, Balzarini J. Deletion of the highly conserved N-glycan at Asn260 of HIV-1 gp120 affects folding and lysosomal degradation of gp120, and results in loss of viral infectivity. PLoS One. 2014 9(6):e101181. Link
Erratum in: PLoS One. 2014 9(10):e110202. Link


Koritzinsky M, Levitin F, van den Beucken T, Rumantir RA, Harding NJ, Chu KC, Boutros PC, Braakman I, Wouters BG. Two phases of disulfide bond formation have differing requirements for oxygen. J Cell Biol. 2013 203(4):615-27. Link
Comment: Short B. A call for oxygen in the ER. J Cell Biol. 2013 203(4): 552.
Comment: Baumann K. Protein folding: A late need for oxygen. Nat Rev Mol Cell Biol 2014 15(5).

Hansen RE, Otsu M, Braakman I, Winther JR. Quantifying Changes in the Cellular Thiol-Disulfide Status during Differentiation of B Cells into Antibody-Secreting Plasma Cells. Int J Cell Biol. 2013 2013:898563. Link

Oka OB, Pringle MA, Schopp IM, Braakman I, Bulleid NJ. ERdj5 is the ER reductase that catalyzes the removal of non-native disulfides and correct folding of the LDL receptor. Mol Cell. 2013 50(6):793-804. Link

Kleizen B, Braakman I. Cell biology. A sweet send-off. Science. 2013 340(6135):930-1. Link

Braakman I, Hebert DN. Protein folding in the endoplasmic reticulum. Cold Spring Harb Perspect Biol. 2013 5(5):a013201. Link

Benham AM, van Lith M, Sitia R, Braakman I. Ero1-PDI interactions, the response to redox flux and the implications for disulfide bond formation in the mammalian endoplasmic reticulum. Philos Trans R Soc Lond B Biol Sci. 2013 368(1617):20110403. Link

Tabak HF, Braakman I, van der Zand A. Peroxisome formation and maintenance are dependent on the endoplasmic reticulum. Annu Rev Biochem. 2013 82:723-44. Link


van der Zand A, Gent J, Braakman I, Tabak HF. Biochemically distinct vesicles from the endoplasmic reticulum fuse to form peroxisomes. Cell. 2012 149(2):397-409. Link
Comment: Schuldt A. Organelle biogenesis: When two become one. Nat Rev Mol Cell Biol. 2012 13(6): 337.
Comment: Goodman C. Cell biology: Peroxisomes come together. Nat Chem Biol. 2012 8: 503.


Peters KW, Okiyoneda T, Balch WE, Braakman I, Brodsky JL, Guggino WB, Penland CM, Pollard HB, Sorscher EJ, Skach WR, Thomas PJ, Lukacs GL, Frizzell RA. CFTR Folding Consortium: methods available for studies of CFTR folding and correction. Methods Mol Biol. 2011 742:335-53. Link

Braakman I, Bulleid NJ. Protein folding and modification in the mammalian endoplasmic reticulum. Annu Rev Biochem. 2011 80:71-99. Link


Hoelen H, Kleizen B, Schmidt A, Richardson J, Charitou P, Thomas PJ, Braakman I. The primary folding defect and rescue of ΔF508 CFTR emerge during translation of the mutant domain. PLoS One. 2010 5(11):e15458. Link

van der Zand A, Braakman I, Tabak HF. Peroxisomal membrane proteins insert into the endoplasmic reticulum. Mol Biol Cell. 2010 21(12):2057-65. Link

Christis C, Fullaondo A, Schildknegt D, Mkrtchian S, Heck AJ, Braakman I. Regulated increase in folding capacity prevents unfolded protein stress in the ER. J Cell Sci. 2010 123(Pt 5):787-94. Link

Pena F, Jansens A, van Zadelhoff G, Braakman I. Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum. J Biol Chem. 2010 285(12):8656-64. Link

Balch WE, Braakman I, Frizzell R, Guggino W, Lukacs G, Penland C, Skach W, Sorscher E, Thomas P, Pollard H. The folding biology of cystic fibrosis: a consortium-based approach to disease. Protein misfolding diseases : current and emerging principles and therapies. 2010. Chapter 19. Link


van Anken E, Pena F, Hafkemeijer N, Christis C, Romijn EP, Grauschopf U, Oorschot VM, Pertel T, Engels S, Ora A, Lástun V, Glockshuber R, Klumperman J, Heck AJ, Luban J, Braakman I. Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1. Proc Natl Acad Sci U S A. 2009 106(40):17019-24. Link

Braakman I. Entering a new era with Ero. Nat Rev Mol Cell Biol. 2009 10(8):503. Link

Bontjer I, Land A, Eggink D, Verkade E, Tuin K, Baldwin C, Pollakis G, Paxton WA, Braakman I, Berkhout B, Sanders RW. Optimization of human immunodeficiency virus type 1 envelope glycoproteins with V1/V2 deleted, using virus evolution. J Virol. 2009 83(1):368-83. Link


Sanders RW, Hsu ST, van Anken E, Liscaljet IM, Dankers M, Bontjer I, Land A, Braakman I, Bonvin AM, Berkhout B. Evolution rescues folding of human immunodeficiency virus-1 envelope glycoprotein GP120 lacking a conserved disulfide bond. Mol Biol Cell. 2008 19(11):4707-16. Link

Christis C, Lubsen NH, Braakman I. Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol. FEBS J. 2008 275(19):4700-27. Link

Braakman I, Otsu M. Biochemistry. Cargo load reduction. Science. 2008 321(5888):499-500. Link

van Anken E, Sanders RW, Liscaljet IM, Land A, Bontjer I, Tillemans S, Nabatov AA, Paxton WA, Berkhout B, Braakman I. Only five of 10 strictly conserved disulfide bonds are essential for folding and eight for function of the HIV-1 envelope glycoprotein. Mol Biol Cell. 2008 19(10):4298-309. Link

Tabak HF, van der Zand A, Braakman I. Peroxisomes: minted by the ER. Curr Opin Cell Biol. 2008 20(4):393-400. Link

Sanders RW, van Anken E, Nabatov AA, Liscaljet IM, Bontjer I, Eggink D, Melchers M, Busser E, Dankers MM, Groot F, Braakman I, Berkhout B, Paxton WA. The carbohydrate at asparagine 386 on HIV-1 gp120 is not essential for protein folding and function but is involved in immune evasion. Retrovirology. 2008 5:10. doi: 10.1186/1742-4690-5-10. Link


Tabak HF, Hoepfner D, van der Zand A, Geuze HJ, Braakman I, Huynen MA. Formation of peroxisomes: present and past. Biochim Biophys Acta. 2006 1763(12):1647-54. Link

van der Zand A, Braakman I, Geuze HJ, Tabak HF. The return of the peroxisome. J Cell Sci. 2006 119(Pt 6):989-94. Link

De Jonge W, Tabak HF, Braakman I. Chaperone proteins and peroxisomal protein import. Top Curr Genet – Chaperones. 2006 Chapter 6:149-83. Link

Piersma D, Berns EM, Verhoef-Post M, Uitterlinden AG, Braakman I, Pols HA, Themmen AP. A common polymorphism renders the luteinizing hormone receptor protein more active by improving signal peptide function and predicts adverse outcome in breast cancer patients. J Clin Endocrinol Metab. 2006 91(4):1470-6. Link


van Anken E, Braakman I. Endoplasmic reticulum stress and the making of a professional secretory cell. Crit Rev Biochem Mol Biol. 2005 40(5):269-83. Link

Kleizen B, van Vlijmen T, de Jonge HR, Braakman I. Folding of CFTR is predominantly cotranslational. Mol Cell. 2005 20(2):277-87. Link

van Anken E, Braakman I. Versatility of the endoplasmic reticulum protein folding factory. Crit Rev Biochem Mol Biol. 2005 40(4):191-228. Link

Hoepfner D, Schildknegt D, Braakman I, Philippsen P, Tabak HF. Contribution of the endoplasmic reticulum to peroxisome formation. Cell. 2005 122(1):85-95. Link
Comment: Schekman R. Peroxisomes: another branch of the secretory pathway? Cell 2005 122:1-2.
Comment: Kunau WH. Peroxisome biogenesis: end of the debate. Curr Biol. 2005 15(18): 774-R776.

Romijn EP, Christis C, Wieffer M, Gouw JW, Fullaondo A, van der Sluijs P, Braakman I, Heck AJ. Expression clustering reveals detailed co-expression patterns of functionally related proteins during B cell differentiation: a proteomic study using a combination of one-dimensional gel electrophoresis, LC-MS/MS, and stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics. 2005 4(9):1297-310. Link

Maggioni MC, Liscaljet IM, Braakman I. A critical step in the folding of influenza virus HA determined with a novel folding assay. Nat Struct Mol Biol. 2005 12(3):258-63.Link

Maggioni C, Braakman I. Synthesis and quality control of viral membrane proteins. Curr Top Microbiol Immunol. 2005 285:175-98. Link

Liscaljet IM, Kleizen B, Braakman I. Studying protein folding in vivo. Protein Folding Handbook. 2005 Part II, Volume 1, Chapter 3: 73-104. Link


Gent J, Braakman I. Low-density lipoprotein receptor structure and folding. Cell Mol Life Sci. 2004 61(19-20):2461-70. Link

Kleizen B, Braakman I. Protein folding and quality control in the endoplasmic reticulum. Curr Opin Cell Biol. 2004 16(4):343-9. Link
Erratum in: Curr Opin Cell Biol. 2004 16(5):597. Link


Sitia R, Braakman I. Quality control in the endoplasmic reticulum protein factory. Nature. 2003 426(6968):891-4. Link

van der Vlies D, Makkinje M, Jansens A, < strong>Braakman I, Verkleij AJ, Wirtz KW, Post JA. Oxidation of ER resident proteins upon oxidative stress: effects of altering cellular redox/antioxidant status and implications for protein maturation. Antioxid Redox Signal. 2003 5(4):381-7. Link

Jansens A, Braakman I. Pulse-chase labeling techniques for the analysis of protein maturation and degradation. Methods Mol Biol. 2003 232:133-45. Link

Tabak HF, Murk JL, Braakman I, Geuze HJ. Peroxisomes start their life in the endoplasmic reticulum. Traffic. 2003 4(8):512-8. Link

Land A, Zonneveld D, Braakman I. Folding of HIV-1 envelope glycoprotein involves extensive isomerization of disulfide bonds and conformation-dependent leader peptide cleavage. FASEB J. 2003 17(9):1058-67. Link

van Anken E, Romijn EP, Maggioni C, Mezghrani A, Sitia R, Braakman I, Heck AJ. Sequential waves of functionally related proteins are expressed when B cells prepare for antibody secretion. Immunity. 2003 18(2):243-53. Link


Jansens A, van Duijn E, Braakman I. Coordinated nonvectorial folding in a newly synthesized multidomain protein. Science. 2002 298(5602):2401-3. Link


Mezghrani A, Fassio A, Benham A, Simmen T, Braakman I, Sitia R. Manipulation of oxidative protein folding and PDI redox state in mammalian cells. EMBO J. 2001 20(22):6288-96. Link

Land A, Braakman I. Folding of the human immunodeficiency virus type 1 envelope glycoprotein in the endoplasmic reticulum. Biochimie. 2001 83(8):783-90. Link

Braakman I. A novel lectin in the secretory pathway. An elegant mechanism for glycoprotein elimination. EMBO Rep. 2001 2(8):666-8. Link

Braakman I, Hebert DN. Analysis of disulfide bond formation. Curr Protoc Protein Sci. 2001 Chapter 14:Unit14.1. Link


Benham AM, Braakman I. Glycoprotein folding in the endoplasmic reticulum. Crit Rev Biochem Mol Biol. 2000 35(6):433-73. Link

Braakman I, van Anken E. Folding of viral envelope glycoproteins in the endoplasmic reticulum. Traffic. 2000 1(7):533-9. Link

Kleizen B, Braakman I, de Jonge HR. Regulated trafficking of the CFTR chloride channel. Eur J Cell Biol. 2000 79(8):544-56. Link

Benham AM, Cabibbo A, Fassio A, Bulleid N, Sitia R, Braakman I. The CXXCXXC motif determines the folding, structure and stability of human Ero1-Lα. EMBO J. 2000 19(17):4493-502. Link

Distel B, Braakman I, Elgersma Y, Tabak HF. Transactions at the peroxisomal membrane. Subcellular Biochemistry – Fusion of biological membranes and related problems. 2000 Chapter 8:303-22. Link

Ermonval M, Duvet S, Zonneveld D, Cacan R, Buttin G, Braakman I. Truncated N-glycans affect protein folding in the ER of CHO-derived mutant cell lines without preventing calnexin binding. Glycobiology. 2000 10(1):77-87. Link


Das AT, Land A, Braakman I, Klaver B, Berkhout B. HIV-1 evolves into a nonsyncytium-inducing virus upon prolonged culture in vitro. Virology. 1999 263(1):55-69. Link

Tabak HF, Braakman I, Distel B. Peroxisomes: simple in function but complex in maintenance. Trends Cell Biol. 1999 9(11):447-53. Link


Hettema EH, Ruigrok CC, Koerkamp MG, van den Berg M, Tabak HF, Distel B, Braakman I. The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import. J Cell Biol. 1998 142(2):421-34. Link

van Weering DH, Moen TC, Braakman I, Baas PD, Bos JL. Expression of the receptor tyrosine kinase Ret on the plasma membrane is dependent on calcium. J Biol Chem. 1998 273(20):12077-81. Link


Zhang JX, Braakman I, Matlack KE, Helenius A. Quality control in the secretory pathway: the role of calreticulin, calnexin and BiP in the retention of glycoproteins with C-terminal truncations. Mol Biol Cell. 1997 8(10):1943-54. Link


Chen W, Helenius J, Braakman I, Helenius A. Cotranslational folding and calnexin binding during glycoprotein synthesis. Proc Natl Acad Sci U S A. 1995 92(14):6229-33. Link


Hammond C, Braakman I, Helenius A. Role of N-linked oligosaccharide recognition, glucose trimming, and calnexin in glycoprotein folding and quality control. Proc Natl Acad Sci U S A. 1994 91(3):913-7. Link


Tatu U, Braakman I, Helenius A. Membrane glycoprotein folding, oligomerization and intracellular transport: effects of dithiothreitol in living cells. EMBO J. 1993 12(5):2151-7. Link

de Silva A, Braakman I, Helenius A. Posttranslational folding of vesicular stomatitis virus G protein in the ER: involvement of noncovalent and covalent complexes. J Cell Biol. 1993 120(3):647-55. Link

Helenius A, Tatu U, Marquardt T, Braakman I. Protein Folding in the Endplasmic Reticulum. Cell Biology and Biotechnology. 1993 Chapter 10: 125-36. Link


Helenius A, Marquardt T, Braakman I. The endoplasmic reticulum as a protein-folding compartment. Trends Cell Biol. 1992 2(8):227-31. Link

Braakman I, Helenius J, Helenius A. Manipulating disulfide bond formation and protein folding in the endoplasmic reticulum. EMBO J. 1992 11(5):1717-22. Link

Braakman I, Helenius J, Helenius A. Role of ATP and disulphide bonds during protein folding in the endoplasmic reticulum. Nature. 1992 356(6366):260-2. Link


Braakman I, Hoover-Litty H, Wagner KR, Helenius A. Folding of influenza hemagglutinin in the endoplasmic reticulum. J Cell Biol. 1991 114(3):401-11. Link

Braakman I, Keij J, Hardonk MJ, Meijer DK, Groothuis GM. Separation of periportal and perivenous rat hepatocytes by fluorescence-activated cell sorting: confirmation with colloidal gold as an exogenous marker. Hepatology. 1991 13(1):73-82. Link


Thalhammer T, Gessl A, Braakman I, Graf J. Separation of hepatocytes of different acinar zones by flow cytometry. Cytometry. 1989 10(6):772-8. Link

Braakman I, Pijning T, Verest O, Weert B, Meijer DK, Groothuis GM. Vesicular uptake system for the cation lucigenin in the rat hepatocyte. Mol Pharmacol. 1989 36(4):537-42. Link

Braakman I, Verest O, Pijning T, Meijer DK, Groothuis GM. Zonal distribution of the cation lucigenin in rat liver: influence of taurocholate. Mol Pharmacol. 1989 36(4):532-6. Link

Braakman I, Groothuis GM, Meijer DK. Zonal compartmentation of perfused rat liver: plasma reappearance of rhodamine B explained. J Pharmacol Exp Ther. 1989 249(3):869-73. Link


van der Sluijs P, Braakman I, Meijer DK, Groothuis GM. Heterogeneous acinar localization of the asialoglycoprotein internalization system in rat hepatocytes. Hepatology. 1988 8(6):1521-9. Link


Braakman I, Groothuis GM, Meijer DK. Acinar redistribution and heterogeneity in transport of the organic cation rhodamine B in rat liver. Hepatology. 1987 7(5):849-55. Link